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Слайды и текст к этой презентации:
№1 слайд![Cell Size](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img0.jpg)
Содержание слайда: Cell Size
№2 слайд![Microscope Measurement How](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img1.jpg)
Содержание слайда: Microscope Measurement
How big is that object in the microscope?
№3 слайд![Lesson Objectives Calculate](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img2.jpg)
Содержание слайда: Lesson Objectives
Calculate the magnification using different objective lens.
Differentiate between eyepiece graticule and the stage micrometer.
Convert mm to micrometers.
Calculate the cell length and breadth using the relationship between the size of the image, actual size and magnification.
The structure and function of different parts of the microscope
The difference between a light microscope and an electron microscope.
№4 слайд![](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img3.jpg)
№5 слайд![](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img4.jpg)
№6 слайд![Light Microscope](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img5.jpg)
Содержание слайда: Light Microscope
№7 слайд![Electron Microscope](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img6.jpg)
Содержание слайда: Electron Microscope
№8 слайд![](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img7.jpg)
№9 слайд![Light microscope vs Electron](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img8.jpg)
Содержание слайда: Light microscope vs Electron microscope
What is the difference between a light microscope and an electron microscope? A number of differences such as the source of light they use, their magnification level, cost, resolving power, among other factors sets these two types of microscopes apart from each other.
VIDEO
№10 слайд![What is happening to the](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img9.jpg)
Содержание слайда: What is happening to the image as you increase the power of the objective lens?
What is happening to the image as you increase the power of the objective lens?
№11 слайд![Calculating total](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img10.jpg)
Содержание слайда: Calculating total magnification
If two lenses are always magnifying the specimen, how do you figure out the total magnification being used ?
Total Magnification = ocular x objective
= 10 x 4 (low power)
= 40 (low power)
№12 слайд![How do we find the overall](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img11.jpg)
Содержание слайда: How do we find the overall magnification of a light microscope?
№13 слайд![](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img12.jpg)
№14 слайд![](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img13.jpg)
№15 слайд![](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img14.jpg)
№16 слайд![The diagram below is a](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img15.jpg)
Содержание слайда: The diagram below is a drawing of an organelle from a ciliated cell as seen with an electron microscope.
The diagram below is a drawing of an organelle from a ciliated cell as seen with an electron microscope.
Calculate the actual length of the organelle as shown by the line AB in the diagram. Express your answer to the nearest micrometer (mm).
Show your working.
Answer = ........................................... mm
№17 слайд![Calculating actual size](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img16.jpg)
Содержание слайда: Calculating actual size:
№18 слайд![To accurately measure the](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img17.jpg)
Содержание слайда: To accurately measure the size of cellular structures we need a suitable scale:
№19 слайд![Field of View When you look](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img18.jpg)
Содержание слайда: Field of View
When you look into a microscope, the “field of view” is the visible circular area.
What happens to your field of view when you increase the power of the objective lens?
By knowing the size of the field of view (diameter), you can measure the size of objects in the microscope.
The size of objects in the field of view is different at each magnification you have to calculate the diameters of the fields of view at each magnification.
This process is called “calibrating your microscope”
№20 слайд![Estimating Specimen Size The](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img19.jpg)
Содержание слайда: Estimating Specimen Size
The area of the slide that you see when you look through a microscope is called the "Field of View".
If you know how wide your field of view is, you can estimate the size of things you see in the field of view.
№21 слайд![Ideally, we need a scale we](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img20.jpg)
Содержание слайда: Ideally, we need a scale we can see directly alongside the cells we are observing:
№22 слайд![Eye piece graticule or](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img21.jpg)
Содержание слайда: Eye piece graticule or reticule
It is a glass or plactic disc with 8 divisions etched on to its surface and fitted into one eyepiece.
The size of the eyepiece reticule is constant despite the change in magnification of the object.
The value of each division varies with the change in magnification.
№23 слайд![Stage Micrometer simply a](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img22.jpg)
Содержание слайда: Stage Micrometer
simply a microscope slide with a finely divided scale marked on the surface.
1 division= 0.01 mm
10 divisions= 0.1 mm
100 divisions = 1 mm
1 mm = 1000 micrometers.
№24 слайд![Instructions Take sample of](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img23.jpg)
Содержание слайда: Instructions
Take sample of onion cell (peel of the onion)
Add a drop of water
Cover the subject glass with cover slip
Fix it with the stage clips.
Focus the specimen on low power objective lens.
Now change to medium power objective lens and observe.
Change to high power objective lens and observe.
№25 слайд![IMPORTANT FORMULA! Object](/documents_6/fb0bac4030f84d36d8dbbaababa162eb/img24.jpg)
Содержание слайда: IMPORTANT FORMULA!
Object Size = field of view (in mm) • 1000
number of “fits”
Object Size = ? µm
** Remember that the field of view changes with each objective!.